State Route 165 (SR 165) is an east to west secondary highway in Monroe County, Tennessee, United States that is 23.8 miles (38.3 km) long.

It is the Tennessee portion of the Cherohala Skyway which runs through Cherokee National Forest and connects Tellico Plains with Robbinsville, North Carolina.

Its western terminus is at SR 68 in Tellico Plains and its eastern terminus is at the North Carolina border, where the road continues as North Carolina Highway 143 (NC 143).

It travels east and straddles the northern edge of downtown to have an intersection with SR 360.

SR 165 then leaves the river to climb the mountains, where it becomes extremely curvy as it gains altitude.