State Route 334 (SR 334), also known as Louisville Road, is 2.5 mile long north-south state highway in Blount County, Tennessee.

It serves as a connector between the town of Louisville and the city of Alcoa.

It heads northwest through suburban areas before entering farmland while passing along the southern edge of McGhee Tyson Airport.

The highway continues northwest through farmland to enter Louisville and come to a roundabout with Miser Station Road and Mentor Road.

The entire route of SR 334 is a two-lane highway.