State Route 354 (SR 354) is a state highway in Washington County, Tennessee.

It connects Jonesborough with Boones Creek.

SR 354 begins at an intersection with US 11E/US 321/SR 34 in Jonesborough and continues north to an intersection with I-26/US 23 in the Boones Creek neighborhood of North Johnson City and ends at an intersection with SR 36 in Boones Creek.

The entire route is in Washington County.

This Tennessee road or road transport-related article is a stub.