[4] In 2019, a controversial Pew Research study stated that belief among U.S. Catholics in the dogma of the Real Presence (Transubstantiation) was as low as 33%, alarming many of the nation's bishops.

[8] Joe Biden's public support of abortion was interpreted by many to put him in a state of grave sin and thus made him ineligible for reception of Communion.

[10] Archbishop José Gómez, president of the United States Conference of Catholic Bishops, informed the Congregation for the Doctrine of the Faith that the bishops were planning on submitting a draft of a document "to address the situation of Catholics in public office who support legislation allowing abortion, euthanasia or other moral evils".

[11] A May 7 response from Luis Ladaria Ferrer, prefect of the Congregation for the Doctrine of the Faith, urged bishops to take an "extensive and serene" look at the topic.

[11] A group of bishops led by Cardinals Blase J. Cupich and Wilton Daniel Gregory pushed for the tabling of the conversation altogether.

[25][1] In the time leading up to the congress, Pope Francis praised the efforts of the organizers and called it "a significant moment in the life of the Church in the United States".

[31] The second day included youth, Spanish, and Extraordinary Form Masses as well as a Byzantine Divine Liturgy celebrated by Bohdan Danylo.

[35] After testimonies by Lila Rose and the parents of Michelle Duppong, the evening ended with adoration led by Bishop William Byrne of the Diocese of Springfield in Massachusetts.

[33][31] Morning session speakers included Tony Meléndez, Mary Healy, Patrick Madrid, and bishops Gustavo García-Siller, Joseph A. Espaillat, and Andrew Cozzens.

[33] Afternoon breakout session topics included Priestly Eucharistic Identity by Bishop Robert Barron, gender dysphoria, and women.

[36] Prior to the keynotes, Costa Rican former professional tennis player Paula Umaña spoke of the healing she credits to prayers and the Blessed Virgin Mary.

[33][37] On July 20, the main liturgy held at Lucas Oil Stadium was a Syro-Malabar Holy Qurbana celebrated by Bishop Joy Alappatt and Archbishop Borys Gudziak.

[33] In the late afternoon, a Eucharistic procession travelled through downtown Indianapolis from the Lucas Oil Stadium to the Indiana World War Memorial, with the final benediction offered by Bishop Andrew Cozzens.

[42] Cardinal Luis Tagle, the papal delegate for the congress, celebrated the closing Mass at Lucas Oil Stadium.

[43] He preached on the need for missionary zeal, and expressed the wishes of the Holy Father that the congress would bear much fruit in the Church and society the United States.

[45] Plans were also announced for a eucharistic pilgrimage to begin in Indianapolis in the spring of 2025, with its conclusion in Los Angeles on the Solemnity of the Most Holy Body and Blood of Christ on June 22, 2025.