Tombusvirus 5′ UTR is an important cis-regulatory region of the Tombus virus genome.

Tomato bushy stunt virus is the prototype member of the Tombusviridae family.

[3] TSD-DSD interactions are proposed to be involved in the mediation of viral RNA replication.

[3] An interesting feature of Tombusvirus is its ability to support the replication of defective interfering (DI) RNAs.

These sub-viral replicons are small, non-coding, deletion mutants of the viral genome that maintain cis-acting RNA elements necessary for replication [1] Other non-coding RNA structures in Tombusvirus include the 3′ UTR region IV[4] and an internal replication element.