The Trojans moved from the NAIA to NCAA Division II in 2012–2013 and were a founding member of the Great Midwest Athletic Conference.

The first intercollegiate team fielded by Trevecca was men's basketball, led by Elmer Heaberlin in 1969 during Dr. Mark R. Moore's tenure as university president.

Men's sports include baseball, basketball, cross country, golf, soccer, and track and field (indoor/outdoor); while women's sports include basketball, cross country, golf, soccer, softball, STUNT, track and field (indoor/outdoor), and volleyball.

The team defeated defending national champion Lipscomb in the District 24 Tournament, then reached the Elite Eight in Kansas City at Kemper Arena.

David Suddeth is the all-time leading scorer in Trevecca basketball history, he scored 3,004 points on the way to earning All-American honors.

Julie Van Beek led the restarted program to the NAIA National Championships in just three seasons and recruited a number of All-American's and Hall of Famers.

He ended up becoming the winningest coach in school history, helping the Trojans transition to NCAA Division II.

Trevecca has a storied baseball program that reached the NCAA Division II College World Series in Cary, NC in 2021.

The last is Hunter Newman (junior, Chapmansboro, Tennessee), an all-American third baseman, was drafted by the St. Louis Cardinals in the 22nd round with the 671st pick.

Rob Erwin, Keith Black, Eric Van Slyke, Luke Brown, Maurice Cole and Jeremy Todd were signed and played professionally.

Stipe Miocic played baseball for Trevecca, the two-time UFC World Heavyweight Champion was on the 2005 Tran South Athletic Conference (TranSouth) championship team coached by Jeff Forehand.