Tris-buffered saline (TBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range.
Tris (with HCl) has a slightly alkaline buffering capacity in the 7–9.2 range.
The pKa declines approximately 0.03 units per degree Celsius rise in temperature.
Additives can be used to add to a compound's functionality.
TBS is often used in immuno-blotting for both membrane washing and antibody dilution.