Twekkelo was traditionally included in the parish of Enschede, along with Lonneker, Driene, Usselo and De Eschmarke.

The heart of Twekkelo is three closely positioned pieces of arable pasture, surrounded by a number of farmsteads, one or two of which were already mentioned in ninth and tenth century records.

Three streams, the Makkenbroekenbeek (Twekkelerbeek), the Strootsbeek (later Elsbeek) and the Schoolbeek flow from east to west through Twekkelo.

A number of walking trails have been established and steps have been taken to make the rural charms of the place better known to outsiders.

The little Johanneskerk was built in 1950 by the "Armenstaat Twekkelo", an organisation set up to serve poor and materially disadvantaged local members of the Dutch Reformed Church.