In endemic regions, Tzanck test is used to diagnose leishmaniasis and leprosy.

For other regions, Tzanck test is mainly used to diagnose pemphigus and herpetic infections.

[4] This is because the advantages of this test are not well known, and the main textbooks of dermatopathology do not include dedicated sections for cytology or Tzanck smear.

[5] A deep learning model called TzanckNet has been developed to lower the experience barrier needed to use this test.

[6] A modified test can be performed using proprietary agents which requires fewer steps and allows the sample to be fixed quicker.