By the time it was decommissioned in 1973, all but the section north of Cygnet ran concurrently with Interstate 75 (I-75).

In 1973, the route was decommissioned in Ohio and Michigan due to its redundancy with Interstate highways.

From Cygnet northward, the independent routing of US 25, up to its concurrency with US 24 at the intersection of Detroit Avenue and Cherry Street, became the whole of SR 25.

The former concurrency to Telegraph Road retained the US 24 designation, while the remaining segment of Detroit Avenue northward to the state line was decommissioned as a state highway, continuing into Michigan as M-125/Dixie Highway.

In 1986, the segment of SR 25 along Detroit Avenue was swapped with US 24, with the state route taking a new alignment from downtown Toledo to I-280.