OB/GYNs Jen Gunter, Chavone Momon-Nelson and Jocelyn Fitzgerald all expressed worry that the advertising campaign will make teens ashamed of their vaginas and that the treatment will cause issues such as irritation and allergic reaction.
Vagisil responded to this criticism explaining that their products have an appropriate pH level for external use in the vulvar area and have been tested by independent dermatologists and gynecologists.
In the South Park episode "Poor and Stupid" Cartman sees a commercial for Vagisil which lists short-term memory loss as a possible side effect.
In the TV show The Big Bang Theory, a Vagisil coupon that came in the mail to Sheldon Cooper appears at the end of the second episode of the seventh season, which he presents as a gift to Penny with a comment: "Think of me when you apply it".
In the movie Major League, aging Starting Pitcher Eddie Harris illegally uses Vagisil to increase the drop on his "Curveball".