Willis Avenue station was opened on November 25, 1886, by the Suburban Rapid Transit Company as a connecting spur to the Harlem River and Port Chester Railroad's Harlem River Terminal Station.
The HR&PC was chartered 20 years earlier and operated trains owned by the New York, New Haven and Hartford Railroad.
The station was located next to the 133rd Street Yard, and served both the Second and Third Avenue line trains.
The station was closed for IRT service on April 14, 1924, when a connecting pedestrian bridge was opened between the nearby Third Avenue El station at 133rd Street,[4] although the Third Avenue Line continued to cross the Harlem River until 1955.
Harlem River Station continued to serve the New Haven Railroad and New York, Westchester and Boston Railway until 1930 when the NYNH&H left, and was closed completely on December 31, 1937, when the NYW&B fell into bankruptcy.