A vast majority of the line remains in service by CSX Transportation, who operates it today as their Bone Valley Subdivision.

Griffin and Winn built the Winston and Bone Valley Railroad to serve the lumber mill.

[2][3] In 1980, the Seaboard Coast Line's parent company merged with the Chessie System, creating the CSX Corporation.

What remains of the line continues to be operated today by CSX Transportation and is designated as their Bone Valley Subdivision.

The line connects to the Valrico Subdivision (which is CSX's busiest route through the Bone Valley) through Mulberry Yard at Prairie Junction.

[5] The Bone Valley Subdivision still serves a number of phosphate mines along its route between Achan and Agricola.