WIS 65 then turns due north to its interchange with Interstate 94 (I-94) near Twin Lakes (Exit 10).

The street is named for the former Wisconsin state governor, Warren P. Knowles, who lived in the city.

WIS 65 continues through the city, becoming North Knowles Avenue after crossing the Willow River.

At its intersection with Hill Avenue (CTH-H), WIS 65 curves to the west for a block before turning north onto Main Street.

WIS 65 continues north through a mostly rural part of the state, finally reaching its northern terminus with US 8 near Deer Lake, east of St. Croix Falls.

As a result, WIS 65 was moved onto a Patch Grove–Monroe route (some parts were not previously signed as a state highway).