Wyoming Highway 316 (WYO 316) is an 11.91-mile-long (19.17 km) east–west Wyoming state road in central Platte County.

Wyoming Highway 316 begins its western end in on the eastern side of Wheatland at I-25 Business/US 87 Business (9th Street).

Highway 316 heads east, named Gilchrist Street, crosses railroad tracks and Rock Creek before passing along the south side of Phifer Airfield on Wheatland's eastern edge.

[1][2] WYO 316 leaves Wheatland at eight-tenths of a mile and passes through the community (CDP) of Chugcreek and changes names to Antelope Gap Road.

Past Chugcreek, WYO 316 crosses Chugwater Creek as it continues east until reaching its end at a junction with Dickinson Hill and Deer Creek Roads east of Wheatland.