He also designed the characters for the video game and anime series Gungrave, and has been working on the manga Blood Blockade Battlefront.

He also was drawn to the work done in Shogakukan's Weekly Shōnen Sunday which included artists Rumiko Takahashi and Fujihiko Hosono.

[1] With the help of a publisher friend, he submitted a Trigun story for the February 1995 issue of the Tokuma Shoten magazine Shōnen Captain, and began regular serialization two months later in April.

Nightow created the characters and story for the Sega/Red Entertainment third-person shooter video game series Gungrave.

The series follows the adventures of a photographer who acquires supernatural visions and gets involved in an organization to fight monsters and terrorists.