The yellow-throated antwren (Myrmotherula ambigua) is a species of bird in subfamily Thamnophilinae of family Thamnophilidae, the "typical antbirds".

Adult males have a black and white streaked face and neck with whitish cheeks.

Adult females have tawny-buff to buff streaks (not white) on the face, head, and upperparts.

[7][8][9] The yellow-throated antwren is found in southwestern Venezuela's Amazonas state, in extreme eastern Colombia's Guainía and Vaupés departments, and in Brazil in the upper basin of the Rio Negro.

[7] The yellow-throated antwren's diet has not been detailed but is known to include insects and probably spiders and other arthropods.

Though there is illegal gold mining even in nominally protected areas, "the region in which this species occurs remains one of those that has been least affected by humans in South America".