Zymography

[citation needed] Zymography also refers to a collection of related, fermented products, considered as a body of work.

For example, beer-making involves the application of top (ale) or bottom fermenting yeast (lager), to produce the desired variety of beer.

The synthesis of the yeast can impact the flavor profile of the beer, i.e. diacetyl (taste or aroma of buttery, butterscotch).

The standard protocol may require modifications depending on the sample enzyme; for instance, D. melanogaster digestive glycosidases generally survive reducing conditions (i.e. the presence of 2-mercaptoethanol or DTT), and to an extent, heating.

Indeed, the separations following heating to 50 °C tend to exhibit a substantial increase in band resolution, without appreciable loss of activity.