On May 2, 2014, the largest operation of Ukrainian army aviation since the beginning of the conflict was carried out, a number of militant checkpoints around Sloviansk were destroyed and the city was besieged.

[9] On June 24, an Mi-8 MT helicopter, under the command of Lieutenant Colonel Andriy Belkin, delivered cargo to the forces in the Sloviansk region and picked up a group of specialists from the Security Service of Ukraine who were installing telecommunications equipment in the ATO zone.

After taking off from Mount Karachun at about 17:10, the helicopter was shot down by separatists using MANPADS killing nine people aboard including two belonging to the brigade, Lieutenant Colonel Belkin Andriy Volodymyrovych and Major Mazunov Ruslan Aleksandrovich.

[19] On 29 May 2019 at 23:27 during a night training flight as part of the exercises "Day-Night", contact was lost with an Mi-8 helicopter of the brigade near the village of Sestryatyn, Rivne Oblast.

As a result of the crash, four crew members including brigade commander Colonel Mazepa Ihor Yaroslavovych, Popenko Vladislav Valeriyovych, Romaniuk Vasyl Myronovych and Datsyuk Pavlo Yuriyovych died.

Colonel Grigoriev Oleksandr Oleksandrovich,[23] Captain Nesteruk Dmytro Mykolayovych and Lieutenant Hnatiuk Vasyl Andriyovych were killed.

[24][25] On 8 March 2022, an Mi-24 helicopter of the brigade was shot down near the village of Kulazhyntsi, Brovary district after successfully destroying multiple Russian vehicles and tanks.

Both pilots: Lieutenant Colonel Marynyak Oleksandr Myroslavovych[26] and Captain Bezzub Ivan Romanovych[27] were killed in the shootdown.