The 1989–90 FA Trophy was the twenty-first season of the FA Trophy.
The teams that given byes to this round are Telford United, Darlington, Kettering Town, Wycombe Wanderers, Boston United, Kidderminster Harriers, Barnet, Runcorn, Macclesfield Town, Yeovil Town, Northwich Victoria, Welling United, Cheltenham Town, Enfield, Sutton United, Altrincham, Fisher Athletic, Merthyr Tydfil, Barrow, Farnborough Town, Aylesbury United, Wealdstone, Dartford, Burton Albion, Hyde United, Wokingham Town, Newcastle Blue Star, Bishop Auckland, Redbridge Forest, Windsor and Eton, Billingham Synthonia and Tow Law Town.