The 1999–2000 Czech Cup was the seventh edition of the annual football knockout tournament organized by the Czech Football Association of the Czech Republic.
44 teams took part in the preliminary round.
74 teams entered the competition at this stage.
Along with the 22 winners from the preliminary round, these teams played 48 matches to qualify for the second round.
Čelákovice were the only team from the fourth level to take part in round 4.