2'-O-methylation

[5] Currently, about 55 2'-O-methylations have been identified in yeast alone and 106 in humans[6] and deposited in RNA Modification Base (RMBase) database.

[7] This modification is able to stabilize the structure of RNA while preventing it from undergoing hydrolysis as the hydroxyl group is replaced.

[2] A technique was developed based on this property called RiboMethSeq to quantify the amount of modifications existing in a sample of rRNA.

Ribosomal RNA exists longer in the cell before degradation so utilizing 2'-O-Met would aid in stabilizing its structure.

The epitranscriptomics of this particular RNA modification occurs post-translation, causing a change in the resulting protein without the DNA being altered.