The 2000 MBNA.com 400 was the 27th stock car race of the 2000 NASCAR Winston Cup Series and the 32nd iteration of the event.

It was also the first NASCAR Winston Cup Series start of future champion Kurt Busch.

Johnny Benson Jr., driving for MB2 Motorsports, would set the fastest time in the session, with a lap of 22.518 and an average speed of 159.872 mph (257.289 km/h).

[3] The final practice session, sometimes referred to as Happy Hour, was held on Saturday, September 23, after the preliminary 2000 MBNA.com 200 NASCAR Busch Series race.

Mark Martin, driving for Roush Racing, would set the fastest time in the session, with a lap of 23.519 and an average speed of 153.067 mph (246.337 km/h).

[4][5] Jeremy Mayfield, driving for Penske-Kranefuss Racing, would win the pole, setting a time of 22.518 and an average speed of 159.872 miles per hour (257.289 km/h) in the first round.