The 2000 Pepsi 400 presented by Meijer was the 22nd stock car race of the 2000 NASCAR Winston Cup Series and the 31st iteration of the event.

[3] Dale Jarrett of Robert Yates Racing would set the fastest time in the session, with a lap of 37.678 and an average speed of 191.092 mph (307.533 km/h).

[3] Andy Houston of PPI Motorsports would set the fastest time in the session, with a lap of 38.066 and an average speed of 189.145 mph (304.399 km/h).

[5] The third and final practice session, sometimes referred to as Happy Hour, was held on Saturday, August 19, after the preliminary 2000 NAPAonline.com 250.

[3] Rusty Wallace of Penske-Kranefuss Racing would set the fastest time in the session, with a lap of 39.085 and an average speed of 184.213 mph (296.462 km/h).

[8] Six drivers would fail to qualify: Geoff Bodine, Darrell Waltrip, Stacy Compton, Kyle Petty, David Keith, and Carl Long.