SC Steinfort and FC Avenir Beggen were relegated to the Division of Honour after finishing 13th and 14th in 2008–09.
They were replaced by Division of Honour 2008–09 champions CS Pétange and runners-up FC Mondercange.
US Rumelange as 12th-placed team had to compete in a single play-off match against 3rd-placed Division of Honour side FC Erpeldange 72.
12th-placed UN Käerjeng 97 competed in a relegation play-offs match against the third-placed team from the Division of Honour, CS Oberkorn, for one spot in next season's competition.
UN Käerjeng 97 retained their spot in the league, beating CS Obercorn 3–1.