The 2009 UEFA Regions' Cup was the sixth UEFA Regions' Cup.

It was held in Croatia and won by the Castile and León team from Spain, which beat Romania's Oltenia 2–1 in the final.

The 29 teams which went straight through to the intermediary round were joined by San Marino, Malta and Turkey's Marmara.

The 32 teams were drawn into eight groups of four, with the following countries hosting each group's matches: The winners of each group qualified for the final tournament.

Croatia was chosen to host the final tournament, with matches being played 15 June to 22 June 2009.