The 2010 AFC Cup was the 7th edition of the competition, played between clubs from nations who are members of the Asian Football Confederation.
Note: while the bracket below shows the entire knockout stage, the draw for the round of 16 matches was determined at the time of the group draw.
The draw for the quarter-finals and beyond was held separately, after the conclusion of the round of 16.
Therefore, the two clubs from Syria, Kuwait, and Thailand may not be drawn with each other in the quarter-finals.
Muangthong United won 2–1 on aggregate.