Five days later, in their final pre-season friendly, The Union played the Tampa Bay Rowdies (a fellow first-year expansion club, albeit in the second-division NASL) and lost 1–0.

Philadelphia began their first Major League Soccer regular season on the road with a nationally televised match against Seattle Sounders FC on March 25, 2010.

Two first-half goals from Brad Evans and Fredy Montero were too much for the ten-man Union, as they went down to defeat 2–0 in their first match, in front of a crowd of over 36,000 rabid Seattle fans—and about 100 hearty Sons of Ben who made the trip.

After the game, the Union released Costa Rican defender David Myrie, and sent Ståhl out on loan to their USL-2 affiliate, Harrisburg.

The Union scored a 3–2 victory with a hat-trick by Sebastian Le Toux in front of a crowd of just under 35,000 at their temporary home, Lincoln Financial Field.

After pulling into a tie thanks to Sebastien Le Toux, the Union again were made to suffer loss from a defensive blunder which led to a game-winning penalty.

The Union went behind early, but showed great attacking flair and were kept scoreless only thanks to the heroics of FC Dallas goalkeeper Kevin Hartman.

[3] Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

Italics indicates MLS Player of the Week Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

[19] Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.