The bottom side at the end of the season get relegated.

Ho Chi Minh City and Thanh Hóa were relegated to the 2010 Vietnam First Division after finishing the 2009 season in the bottom two places.

The two relegated teams were replaced by 2009 Vietnam First Division champions XM The Vissai Ninh Bình and runners-up Hòa Phát Hà Nội.

Nam Định defeated Thành phố Cần Thơ in the end of season promotion/relegation match to secure their place in the V. League.

Thể Công and Quân Khu 4 were renamed to Viettel F.C.