Defending champion Serena Williams defeated Vera Zvonareva in the final, 6–3, 6–2 to win the ladies' singles tennis title at the 2010 Wimbledon Championships.

[3] Petra Kvitová (ranked 62nd) and Tsvetana Pironkova (ranked 82nd) reached the semifinals, marking the first time since 1999 that two unseeded players reached the semifinals.

Pironkova became the first Bulgarian woman to reach a major semifinal.

[a][4] Click on the seed number of a player to go to their draw section.

They were replaced in the draw by the highest-ranked non-seeded players Melanie Oudin and Kateryna Bondarenko, who became the #33 and #34 seeds.