[2] The draw of the tournament was held on 12 July 2016, 20:00 CLT (UTC−4), at the Espacio Riesco Convention and Events Center in Huechuraba, Chile.
If still tied, extra time would not be played, and the penalty shoot-out would be used to determine the winner (Regulations Article 5.1).
Tied 1–1 on aggregate, Sportivo Luqueño won on away goals and advanced to the second stage (Match O16).
Tied 1–1 on aggregate, Blooming won on penalties and advanced to the second stage (Match O6).
Tied 2–2 on aggregate, Sol de América won on penalties and advanced to the second stage (Match O13).
Tied 0–0 on aggregate, Montevideo Wanderers won on penalties and advanced to the second stage (Match O11).
Tied 2–2 on aggregate, Real Garcilaso won on away goals and advanced to the second stage (Match O10).
Tied 2–2 on aggregate, Sport Huancayo won on away goals and advanced to the second stage (Match O13).