The 2016 NCAA Division II men's basketball tournament involved 64 teams playing in a single-elimination tournament to determine the national champion of men's NCAA Division II college basketball.

The eight regional winners met in the Elite Eight for the quarterfinal, semifinal, and championship rounds.

Augustana (SD) defeated Lincoln Memorial, 90-81, to win the first national championship in the school's history.

[1] Lubbock Christian, Saginaw Valley State and St. Thomas Aquinas all made their first Division II tournament appearance.

At the same time, Kentucky Wesleyan made its 38th appearance, Philadelphia its 35th, and Seattle Pacific its 26th, but all lost their opening round game.