The season saw the 49ers attempting to rebound from their 5–11 record the previous year, but finished 2–14, with their only wins coming against their division rival Los Angeles Rams.

Running backs (RB) Wide receivers (WR) Tight ends (TE) Defensive linemen (DL) Defensive backs (DB) Special teams Reserve The 49ers' preseason opponents and schedule were announced April 7, 2016. at Levi's Stadium, Santa Clara, California First quarter Second quarter Third quarter Fourth quarter Top passers Top rushers Top receivers

The 49ers wore their black alternate uniforms for this game as part of Color Rush.

Days prior to Week 6, Head Coach Chip Kelly confirmed that quarterback Colin Kaepernick would start in place of Blaine Gabbert.

An attempted rally by the 49ers was stopped short of the end zone in the final seconds.

With the win, the 49ers snapped their 13-game losing streak and swept the Rams on the season, while going 0–13 against the rest of the league.

With the Cleveland Browns standing 0–14 before this week, the 49ers win also ended the possibility of two teams finishing 1–15 for the first time, or becoming the first team since the 2001 Carolina Panthers to win their opener and lose the remainder of their games.