The 2020–21 season is Guadalajara's fourth competitive season and fourth season in the Liga MX Femenil, the top flight of Mexican women's football.

Due to the COVID-19 pandemic, Liga MX Femenil's start was delayed for August 2020.

[1] On 23 July 2020, manager Ramón Villa Zevallos was fired due to differences between him and the club's management, regarding the "players' development".

[2] He was replaced by Édgar Mejía, who previously played for Guadalajara and achieved a league championship in 2006.

[3] Source: Competitions