The chairman Jaroslav Tvrdík also set goals for the upcoming season, a championship three-peat (winning the title for a third consecutive time) and a 2020–21 UEFA Champions League group-stage presence.

[7] On 18 March, Slavia defender Ondřej Kúdela was accused of racially abusing Rangers midfielder Glen Kamara during a Europa League knockout match.

[12] On 24 July, Tomáš Souček completed a permanent transfer to West Ham United where he had been on loan since January 2020,[13] and Josef Hušbauer signed with Anorthosis Famagusta.

[14] On 27 July, Slavia announced the signing of Michal Beran and Tomáš Malinský from Slovan Liberec, to a four-year and three-year contract respectively.

[21] On 18 August, coach Jindřich Trpišovský has extended his contract by three years, until the summer of 2023 with an option for a two-year renewal.

[24] On the same day, Jan Sýkora left the team after a three-year spell for the Polish Ekstraklasa runner-up Lech Poznan.

FC Slovácko on a season-long loan deal, with Slavia retaining the right to recall him in the winter transfer window.

FC Slovácko ended and Jan Stejskal and Jakub Markovič both had their loans cut short.

[37] On 1 February, Petar Musa joined Union Berlin on loan for the remainder of the season,[38] whilst Simon Deli returned to Slavia on a similar deal from Club Brugge.

[39] Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.