Newry City were the champions of the previous season and promoted to the 2022–23 NIFL Premiership.
They were replaced by Warrenpoint Town, who were relegated from the 2021–22 NIFL Premiership.
Queen's University were relegated to the 2022–23 NIFL Premier Intermediate League and replaced by Newington, the champions of the 2021–22 NIFL Premier Intermediate League.
The eleventh-placed club (Institute) were expected to face the second-placed club from the 2022–23 NIFL Premier Intermediate League (Ballymacash Rangers) for one place in the following season's Championship.
However, after Warrenpoint Town were disqualified and expelled from the NIFL Championship, Institute were reprieved from the play-off and replaced by the bottom-placed NIFL Championship club, Knockbreda.