It was part of the garrison of Mobile, Alabama between August 1862 and April 1863, when it was sent to Tullahoma to join the Army of Tennessee.

The regiment participated in the retreat of the army during the Tullahoma campaign and fought in the Battle of Chickamauga, in which it lost 125 men killed and wounded.

The 36th Alabama lost roughly 300 men in action after departing Dalton, and at the Battle of Jonesboro was involved in particularly heavy fighting, losing a quarter of those present.

[1] The regiment and the rest of James T. Holtzclaw's brigade was sent to Mobile to garrison Spanish Fort.

Since the beginning of the Atlanta Campaign, where it had mustered 460 effectives, the regiment had lost 470 men and 21 officers, mostly killed and wounded.