He was born in Grabow as the posthumous son of Duke Adolf Frederick I of Mecklenburg and his second wife, Maria Katharina of Brunswick-Dannenberg (1616–1665).

Frederick William took the Duchy of Mecklenburg-Schwerin, and Adolphus obtained the Principality of Ratzeburg and the Lordship of Stargard with the commanderies of Mirow and Nemerow, of which he formed the Duchy of Mecklenburg-Strelitz.

They had five children:[4] On 20 June 1702, Adolphus Frederick II married secondly to Princess Johanna of Saxe-Gotha (1 October 1680 – 9 July 1704), a daughter of Frederick I, Duke of Saxe-Gotha-Altenburg, and Magdalena Sybille of Saxe-Weissenfels, who was his half-niece.

[4] On 10 June 1705 at Neustrelitz, Adolphus Frederick II married thirdly to Princess Christiane Emilie of Schwarzburg-Sondershausen (13 March 1681 – 1 November 1751), a daughter of Christian William I, Prince of Schwarzburg-Sondershausen and Countess Antonie Sybille of Barby-Mühlingen (1641–1684).

They had two children:[4] Through his granddaughter Charlotte, Adolphus Frederick is the ancestor of every British monarch beginning with George IV, who ascended the throne of the United Kingdom in 1820.