Duke Charles Louis Frederick of Mecklenburg-Strelitz (23 February 1708 – 4 June 1752) was a member of the Strelitz branch of the House of Mecklenburg.

Charles was born in Strelitz, the second son and youngest child of Adolphus Frederick II, Duke of Mecklenburg-Strelitz (1658–1708).

His half-brother succeeded their father as Adolphus Frederick III, whereas Charles, as the younger son, inherited the commanderies of Mirow and Nemerow.

[3] After visiting Geneva, Italy and France, he went to Vienna and briefly entered the service of the Holy Roman Emperor as a Lieutenant Colonel before returning to Mirow.

[2] After leaving the Army, Charles lived with his family in the castle of Mirow, managing his estates and attending to the education of his children.