Her father's second wife, also called Agnes, was the daughter of Conrad II, Margrave of Lusatia.

Agnes' older sister was Irmgard, wife of Herman V, Margrave of Baden-Baden and her brother was Henry VI, Count Palatine of the Rhine.

Since that time also the lion has become a heraldic symbol in the coat-of-arms for Bavaria and the Palatinate.

After a dispute with Emperor Frederick II was ended, Otto joined the Hohenstaufen party in 1241.

Their daughter, Elizabeth, was married to Frederick's son Conrad IV.