Margaret's maternal grandparents were Wenceslaus II of Bohemia and his first wife, Judith of Habsburg.
Her paternal grandparents were, Henry VII, Holy Roman Emperor and his wife Margaret of Brabant.
Her younger brother was crowned Charles IV, Holy Roman Emperor in 1349, and her other siblings included Bonne, wife of John II of France; John Henry, Margrave of Moravia; and Anna, wife of Otto, Duke of Austria.
worsened the relationship between the emperor and Henry XIV, who allied with Margaret's father, John.
Some months after the reconciliation with Louis IV, in February 1339, Henry died from leprosy.