Anzalone originally committed to Ohio State and Notre Dame but chose to play college football at University of Florida from 2013 to 2016.

He finished with two sacks, 59 total tackles (45 solo), one interception, two passes defended, and three forced fumbles.

He finished with 1.5 sacks, 125 total tackles (77 solo), one interception, six passes defended, and one fumble recovery.

[22] In the 2023 season, Anzalone finished with three sacks, 129 total tackles (85 solo), six passes defended, and one fumble recovery in 16 games and starts.

[24] In the 2024 season Anzalone suffered a broken left forearm in the Week 11's 52-6 victory against Jacksonville Jaguars.

Expected to miss 6-8 weeks due to the injury, he was placed on injured reserve on November 18 with hopes of returning in the post-season.

[25] Prior to the injury, Anzalone totaled 56 stops, including seven tackles for loss, and four pass deflections.