[2] Modeling changes in species breeding patterns due to climate as well as understanding the genetic mechanisms that control it has proven to be important.
For example, a species with multiple breeding seasons in a year may shift those times depending on external conditions such as temperature or predation.
The time frames involving allochrony are typically divided into three categories (prevalence in nature as well as examples are provided alongside each category):[2] Other phenotypic traits are often found to co-occur with reproductive timing such as flowering number, egg-clutch sizes, reproductive lifespans, or body size—what can be defined as temporal phenotypic clines.
[4] If heritable, the same factors may be expressed as they are in a plastic explanation; however, gene flow limitations allow for adaptation to the specific conditions of the reproductive time.
[4] Adaptation by time is an extension of divergence due to limited gene flow between populations experiencing different selective pressures.
[13] Typically this is limited to spatial variation such as in ecological speciation; however, in allochrony, selection varies not just in space, but in reproductive time—giving rise to adaptive temporal clines in phenotypic traits that are heritable.
Isolation by time effectively allows adaptive temporal clines to evolve as long as the reproductive season has selective variation.
[14] Testing whether or not allochrony prevents gene flow can be difficult due to the multitude of unknown variables in wild populations and the inability to replicate and manipulate it in laboratory settings.
[1]: 203 Producing viable, and fertile offspring (or the lack thereof) is not always possible; fortunately, lake of mate tests do not necessarily indicate temporal isolation is not at play.
[1]: 206 Determining if allochrony is the source of divergence require a key pattern to be measured: isolation (and subsequently speciation) should correlate with a decrease in overlapping breeding times.
[2] This pattern indicates that daily allochrony is more prone to gene flow (closeness of breeding times can allow accidental intermixing of populations) while yearly allochrony is the least prone to gene flow (accidental intermixing is rare if large time frames exist between mating periods).
[100] In high-latitude regions, various taxa experience similar temperatures and solar radiation in cyclic patterns due to Earth's axial tilt—generating seasons that are not found at the equator.
[100] In contrast with this, latitudes near or at the equator (tropics) experience asynchrony in seasonal variation in that the regions receive similar amounts of solar radiation and maintain consistence temperature.
[100] A large scale test of the hypothesis was conducted on fifty-seven New World bird species across South, Central, and North America.
[2] The findings, using DNA, geographic and ecological distances, as well as climatic data, indicated that genetic differentiation increased in species populations where asynchrony in precipitation was present.