He played college football for the UCF Knights and was selected by the New England Patriots in the fourth round of the 2003 NFL draft.

[2] Ten minutes before kickoff of Samuel's final high school game, he replaced his team's injured starting quarterback.

He threw two touchdown passes, intercepted two more, rushed for 80 yards, and even worked on special teams returning kicks and punts.

[2] Samuel attended the University of Central Florida, majored in business administration, and played for the UCF Knights football team.

[3] He intercepted a pass and returned it for a touchdown twice in the 2006 NFL Playoffs: first against the New York Jets to clinch their Wild Card Round victory and then in the AFC Championship Game against the Indianapolis Colts to extend the lead to 21–3.

After holding out for most of the preseason, Samuel signed the one-year $7.79 million tender on August 27, 2007, to fulfill his obligation under the franchise tag.

According to The Boston Globe, if Samuel participated in 60% of the defensive snaps or the Patriots win 12 games, his contract stipulated that the team would not place the franchise tag on him for the following season.

Late in the game, Samuel dropped a pass thrown by Eli Manning that would have sealed up the Super Bowl for the Patriots.

On the next play, Manning completed a pass to David Tyree known as the Helmet Catch that led to an upset.

Within minutes of the start of free agency on February 29, 2008, Samuel was reportedly already setting up a meeting with the Philadelphia Eagles.

Upon being signed, Samuel was quoted as saying, “I just want to be able to win and get back to the Super Bowl.” On August 22, 2008, he made his return to New England during the third week of the preseason.

Asante Samuel made his regular season Eagles debut on September 7, 2008, against the St. Louis Rams.