[1] Axenic culture is an important tool for the study of symbiotic and parasitic organisms in a controlled environment.
One standard approach with microorganisms is to spread a sample of the culture onto an agar plate, and to incubate this for a fixed period of time.
Such "contaminating" organisms will grow on the plate during this period, identifying cultures that are no longer axenic.
In the case of an asexual species derived from a single individual, the resulting culture should consist of identical organisms (though processes such as mutation and horizontal gene transfer may introduce a degree of variability).
The axenic culture of some pathogens is complicated because they normally thrive within host tissues which exhibit properties that are difficult to replicate in vitro.