The Ayot Greenway is a rail trail which extends from Welwyn Garden City to Wheathampstead in Hertfordshire.

Part of National Cycle Network Route 57, it runs for four miles (with a break where the A1(M) has been constructed) along a former branch line from Welwyn Garden City railway station towards Luton and Dunstable.

Today, there are still visual reminders of the line, with two bridges still intact, the original fencing and even part of a collapsed railway signal.

Walkers and cyclists then use a road bridge over the A1(M) and pick up the trail again just north of Ayot St Peter at what was its railway station.

The original line is bisected by the B653 Wheathampstead bypass on the road to Luton, so the Ayot Greenway forks left through an underpass.