Azobenzene reductase also known as azoreductase (EC 1.7.1.6) is an enzyme that catalyzes the chemical reaction: The 3 substrates of this enzyme are N,N-dimethyl-1,4-phenylenediamine, aniline, and nicotinamide adenine dinucleotide phosphate ion, whereas its 3 products are 4-(dimethylamino)azobenzene, nicotinamide adenine dinucleotide phosphate, and hydrogen ion.
[1] This enzyme belongs to the family of oxidoreductases, specifically those acting on other nitrogenous compounds as donors with NAD+ or NADP+ as acceptor.
The reaction catalyzed by this enzyme proceeds via a ping-pong mechanism[2] by using 2 equivalents of NAD(P)H to reduce one equivalent of the azo compound substrate (for example methyl red where Ar = p-dimethylaniline and Ar' = o-benzoic acid) into two equivalents of aniline product: Most azoreductase isoenzymes can reduce methyl red, but are not able to reduce sulfonated azo dyes.
Other names in common use include: As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1NNI, 1V4B, and 2D5I.
Please check the last updated data on RCSB PDB site.