The main hospital was then closed down, with the building on Haneviim (Prophets') Street, the maternity ward, which serves the residents of the nearby neighborhoods and various clinics, continuing to operate.

In 1864, another complex of buildings was acquired incorporating treatment rooms, a pharmacy, a hospice for the terminally ill and administrative offices.

The Ashkenazi Perushim Hospital, as it was known, became the favorite charity of the British Jewish philanthropist Moses Montefiore, who described the facility in his diary in 1875.

The alley where the hospital stood between 1864 and 1947 is now named after it: Bikur Holim Street, known in Arabic as Ṭarīq Ḥāret ash-Sharaf ('Sharaf Quarter Road').

[6] In 1898, during his visit to Jerusalem, German emperor Wilhelm II donated a large amount of money used for purchasing the plot of land on which the new hospital was built, on what is now Nathan Straus Street.

The building on Chancellor Avenue (now Straus Street), just off Jaffa Road, was completed in 1925 and opened its doors to all residents of Jerusalem, Jews and non-Jews.

Jewish underground fighters were hospitalized under fictitious names to keep the British mandatory police from finding them.

[10] Today, as part of Bikur Cholim Hospital which is being operated as a branch of Shaare Zedek Medical Center, it houses the maternity ward,[11] with the department for gynecological and obstetric endocrinology.

"[3] Situated near the religious neighborhoods of Geula and Mea Shearim, Bikur Holim used to admit a very high percentage of Haredi Jews, and tried to cater to their needs.

[5] The main wing of the current building was designed by architect Zvi Joseph Barsky in the neo-classical style with modernist elements.