BA25 is located in the cingulate region as a narrow band in the caudal portion of the subcallosal area adjacent to the paraterminal gyrus.

[2] This region is extremely rich in serotonin transporters and is considered as a governor for a vast network involving areas like hypothalamus and brain stem, which influences changes in appetite and sleep; the amygdala and insula, which affect the mood and anxiety; the hippocampus, which plays an important role in memory formation; and some parts of the frontal cortex responsible for self-esteem.

[4][5] The subcallosal cingulate gyrus CG25 which consists of BA25 as well as parts of BA24 and BA32 has been implicated as playing an important role in major depression and has been the target of deep brain stimulation to treat the disorder.

[7] A different study found that metabolic hyperactivity in this area is associated with poor therapeutic response of persons with major depressive disorder to cognitive-behavioral therapy and venlafaxine.

[9] In 2013, Walker and Lawson conducted neurofeedback research with 183 subjects experiencing drug resistant depression.