[2] Cannabidiolic acid synthase consists of a single protein with a molecular mass of 74 kDa.
[1] Its amino acid sequence is partly (40-50%) homologous to several other oxidoreductases,[2] such as berberine bridge enzyme in Eschscholzia californica[3] and Nectarin V in Nicotiana langsdorffii X N.
[4] CBDA synthase has four binding sites; two for FAD and two for the substrate.
[5] Cannabidiolic acid synthase catalyses the production of cannabidiolate predominantly from cannabigerolate by stereospecific oxidative cyclization of the geranyl group of cannabigerolic acid[2] according to the following chemical reaction: Cannabinerolate can also be used as a substrate, but with lower efficiency (KM=0.137 mM) than cannabigerolate (KM=0.206 mM).
It covalently binds FAD, and does require coenzymes & molecular oxygen for the oxidocyclization reaction.